Mobility measurement by analysis of fluorescence photobleaching recovery kinetics
نویسندگان
چکیده
منابع مشابه
Mobility of Adsorbed Proteins Studied by Fluorescence Recovery after Photobleaching
The mobility of proteins adsorbed on a solid substrate and on a lipid monolayer was measured by fluorescence recovery after photobleaching. Both a conventional fluorescence microscope with a chargecoupled device camera and a laser scanning confocal microscope were used. For proteins adsorbed on a solid substrate, the bleached area never recovered fully, while for proteins adsorbed at liquid int...
متن کاملAnalysis of Molecular Mobility by Fluorescence Recovery After Photobleaching in Living Cells
1 Centre d'Imagerie Cellulaire et de Cytomètrie (CICC), Centre de Recherche des Cordeliers, Université Pierre et Marie Curie – Paris6, UMR S 872, Paris, F-75006 France. 2 Université Paris Descartes, UMR S 872, Paris, F-75006 France. 3 INSERM, U872, Paris, F-75006 France. 4 Cell and Tissue Imaging Facility (PICT-IBiSA), Institut Curie-CNRS UMR144, Paris, France. 5 RTmfm (Multidimensional Fluores...
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Fluorescence recovery after photobleaching (FRAP) has been a powerful tool for characterizing the mobility of cell surface membrane proteins. However, the application of FRAP to the study of intracellular membrane proteins has been hampered by the lack of specific probes and their physical inaccessibility in the cytoplasm. We have measured the mobility of a model transmembrane protein, the temp...
متن کاملInference of protein kinetics by stochastic modeling and simulation of fluorescence recovery after photobleaching experiments
MOTIVATION Fluorescence recovery after photobleaching (FRAP) is a functional live cell imaging technique that permits the exploration of protein dynamics in living cells. To extract kinetic parameters from FRAP data, a number of analytical models have been developed. Simplifications are inherent in these models, which may lead to inexhaustive or inaccurate exploitation of the experimental data....
متن کاملMobility of microinjected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recovery.
Rhodamine-labeled actin microinjected into living embryonic chicken gizzard cells became associated with its characteristic cytoskeletal structures. In these domains the translational diffusion coefficients (D) of rh-actin were determined in vivo by fluorescence photobleaching recovery (FPR) measurements. Two classes of actin molecules with respect to its mobilities were detected: rh-actin with...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1976
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(76)85755-4